FIG 8.

Effects of MGBG on CCR5 and CD4 expression in macrophages. Pooled monocytes from 6 healthy donors were differentiated in vitro with or without MGBG treatment for 7 days. Cells were harvested and stained for CCR5, CD4, and CD14 for FACS analysis. (A) Two distinct populations of cells were gated based on forward and side scatters (FSC and SSC, respectively). (B) MGBG treatment showed no apparent effects on CCR5 or CD4 expression in the CD14⁻ macrophages. In the CD14^Dim cells, MGBG at 0.14 μM, the highest concentration tested, caused an observable increase in CD4 but a slight decrease in CCR5 expression.