FIG 9.

Temperature-sensitive inhibition of HIV-1 expression by MGBG in macrophages. In vitro differentiated human macrophages were infected with HIV-1 and treated with or without 1.4 μM MGBG for 6 h at either 37 or 4°C as indicated. After drug removal, the cells were cultured for 7 more days before harvested for both EGFP and p24 measurements. The experiments were set up in duplicate using cells from a single donor. Detailed FACS data for EGFP expression are shown in Fig. S6 in the supplemental material.