FIG 4.

Selected mutations in NS3 (3/VA) and NS5B (5B/EG) do not show an impact on RNA replication. (A) Scheme of the bicistronic reporter replicon Bici RLuc NS2-3′. (B) MDBK cells were electroporated with 1 μg of the corresponding RNAs, and luciferase activity was determined at 2, 24, and 48 h pe. Measurements were carried out in triplicates, and experiments were repeated at least 3 times. Data from one representative experiment is shown. Error bars represent standard deviations. Control, no RNA electroporated; GAA, NS5B mutation, replication deficient; WT, wild type; RLUs, relative light units; RLuc, Renilla luciferase.