TABLE 5.
Functional analysis of amino acid permutations at position 1721 in NS3a
| aa V1721 (NS3) change | aa characteristic(s) | RNA replication | Viral titer 48 h peb (TCID50/ml) |
|---|---|---|---|
| Alanine | Nonpolar/hydrophobic | + | 5.0 × 105 |
| Isoleucine | Nonpolar/hydrophobic | + | — |
| Methionine | Nonpolar/hydrophobic | + | — |
| Tyrosine | Polar/neutral | + | — |
| Glutamine | Polar/neutral | + | — |
| Serine | Polar/neutral | + | 1.7 × 105 |
| Glutamic acid | Acidic | − | ND |
| Arginine | Basic | − | ND |
a
Amino acids indicated were used to alter valine 1721 in the context of N7-2/EV-Ubi-3/VA. Data shown are from one representative experiment out of 3 independent experiments.
b
A dash indicates no NS3-positive cells or infectious viruses were detected at 48 h pe. Virus derivatives with no detectable RNA replication were not analyzed for viral titers. ND, not determined.