Skip to main content
. 2015 Sep 2;89(22):11396–11405. doi: 10.1128/JVI.02151-15

FIG 5.

FIG 5

ΔNp73α and p65 inversely regulate TLR9 expression. (A) HPV38 E6/E7 HFK were cotransfected with 1 μg of ΔNp73α S or AS oligonucleotides and 50 pmol of a control siRNA (scramble) or siRNA specific for p65 (sip65). After 36 h, total RNA was extracted and analyzed by RT-qPCR. Data are the means from three independent experiments. **, P < 0.01. (B) HPV38 E6/E7 HFK were transduced with pBabe or pBabe-ΔN-IκBα superrepressor (ΔN-IκBα). Total RNA was extracted and TLR9 levels were analyzed by RT-qPCR. Data are the means from three independent experiments. **, P < 0.01. (C) HPV38 E6/E7 HFK were transfected with scramble or p65 siRNA (left) or with scramble or IKKβ siRNA (right). After 40 h, total RNA was extracted and TLR9 mRNA levels were analyzed by RT-qPCR. Data are the means from three independent experiments. *, P < 0.05; **, P < 0.01.