TABLE 1.
Sequences of different siRNAs used for gene silencing
| Target | siRNA sequence or description (source) |
|---|---|
| Scrambled (negative control) | 5′-GGUGGAAGAGGUGGUGAGC-3′ |
| IKKβ | 5′-CGUACGCGGAAUACUUCGA-3′ |
| EZH2 | 5′-AGUGGUGCUGAAGCCUCAAUGUUUA-3′ |
| p65 | siGenome SMART pool M-003533-02-0005, human RELA, NM_021975 (Thermo Scientific) |
| p38 (MAPK14) | Silencer Select predesigned siRNA, catalog no. 4392420, IDa s3587 (Life Technologies) |
| S ΔNp73α | 5′-accgACGTACAGcatg-3′b |
| AS ΔNp73α | 5′-ccatGCTGTACGtcggT-3′b |
a
ID, identification number.
b
For the ΔNp73α sense (S) and antisense (AS) oligonucleotides, the capital letters indicate phosphorothioate nucleotides.