FIG 4.

Protein levels and distribution of RRP1B in IAV-infected cells. (A) A549 cells were infected (+) or not (−) with influenza A/WSN/33 virus at an MOI of 5 for 3 h and then harvested for Western blot analysis with antibodies against RRP1B and NP. Actin was used as a loading control. (B and C) A549 cells were transduced with lentiviruses carrying Flag-tagged RRP1B cDNA. The RRP1B-Flag-overexpressing A549 cells were infected with influenza A/WSN/33 virus at an MOI of 5. At 3 h p.i., the cells were processed for immunofluorescence with anti-Flag antibody (green), anti-nucleolin antibody (B), or anti-NP antibody (C) (red), and DAPI.