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. 2015 Jul;44(7):987–996.

Fig. 5:

Fig. 5:

Analysis of recombinant hAm expression by using the specific polyclonal antibody to hAmin western blot test. lane 1, non-transfected cell lysate; lane 2, transfected cell lysate; lane 3, protein size marker in kDa.; lane 4, purified rhAm from cell lysate by using Ni-NTA His•Bind® resins, lane 5, positive control (Emdogaine) for western blot test; lane 6, supernatant of cell culture related to transfected I.L.L.; lane 7, supernatant of cell culture related to non-transfected I.L.L