FIG. 4.

Western blots of mouse testicular (a–e) and sperm (f–h) protein extracts treated with deglycosidases (+) or buffer alone (−) and stained with antibodies to SPESP1: PNGase-F (a and f), neuraminidase (b and g), endo-α-N-acetylgalactosaminidase (c), combination of PNGase-F and neuraminidase (d and h), and mixture of deglycosidases (e). Deglycosylated testicular SPESP1 showed major mass shifts, particularly with mixtures of deglycosidases (e), indicating testicular SPESP1 is highly glycosylated.