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Western blot analysis of intra-acrosomal proteins SPESP1, SLLP1, and SAMP14 during capacitation. Noncapacitated (0 h, lane 1), capacitated (90 min, lane 2) and capacitated, ionophore (A23187)-treated, and acrosome-reacted (for 30 min, lane 3) sperm were analyzed with antibodies to SPESP1 (a), SLLP1 (b), SAMP14 (c), and equal loading control beta-tubulin (d). A 27-kDa SPESP1 peptide appeared after capacitation only in SPESP1 samples. S, standard.
