TABLE 1.
Strains and primers used
| Primers or strain | Sequence or description | Source or Ref. |
|---|---|---|
| S1 | CACCGCACAATACAGCAGGGT | This study |
| S2 | CAGAGGATCAAGGGGAAACG | This study |
| S3 | CACCTAAAATCCTGAACTGACC | This study |
| S4 | ATTGGATAGCAGGGAACCAG | This study |
| SgfI-S5 | GCGATCGCACTTGGGGGAAAAA | This study |
| PacI-S6 | TTAATTAACCATATTTTAGCAAATATACCTTAGGAAG | This study |
| HY | ATTGTTGGAGCCGAAATCC | This study |
| YG | AAGATCGTTATGTTTATCGGCACT | This study |
| S-ext1 | TGATCGCACTTGGGGGAAAAA | This study |
| S-ext4 | CATGGGCGGAGTTGGTTTCA | This study |
| S-RT sense | CAACCCGGCTCTAGCTGTTC | This study |
| S-RT antisense | TGAACCACGTAATGATCCGGC | This study |
| S-E216A-sense | TCGTGTTCGCAGCAACGTA | This study |
| S-E216A-antisense | CGTTGCTGCGAACACGA | This study |
| LE | GTTGACCAGTGCCGTTCC | This study |
| BL | GCTGATGAACAGGGTCACG | This study |
| T-trpC | AGAGCGGATTCCTCAGTCTC | This study |
| Sph3 52start | GGGCATATGTCCAAGGTCTTTGTGCCTCTCTATGTG | This study |
| Sph3 298stop | GGCTCGAGCTATTTTCCCATCAAATCCACAAACTC | This study |
| E222A Fwd | GGGCATATGTACAAGGTGGACATGCTCC | This study |
| E222A Rev | GGGCATATGCCCGATCCCGACGACGGCCT | This study |
| E222Q Fwd | CGTTGTTTTTCAAGCAACCTATGC | This study |
| E222Q Rev | GCATAGGTTGCTTGAAAAACAACG | This study |
| D166A Fwd | CTTTTTTGCAGAAACACCGCAGCAG | This study |
| D166A Rev | GTGTTTCTGCAAAAAAGATACCACG | This study |
| D166N Fwd | CTTTTTTAATGAAACACCGCAGCAG | This study |
| D166N Rev | GTGTTTCATTAAAAAAGATACCACG | This study |
| E167A Fwd | GTATCTTTTTTGATGCAACACCGCAG | This study |
| E167A Rev | CTGCGGTGTTGCATCAAAAAAGATAC | This study |
| E216A Fwd | CGTTCGTGTTCGCAGCAACGTACG | This study |
| E216A Rev | GGTGTCGTACGTTGCTGCGAACAC | This study |
| Af293 | Wild-type pathogenic strain of A. fumigatus | P. Magee |
| B834 Met− | E. coli laboratory expression strain: F− ompT hsdSB (rB− mB−) gal dcm met (DE3) | Novagen |
| TOP10 | E. coli cloning strain | Invitrogen |
| BL21 CodonPlus | E. coli laboratory expression strain: F− ompT hsdS (rB− mB−) dcm+ Tetr galλ (DE3) endA [argU proL Camr] | Stratagene |