FIGURE 6.

Affinity-purified anti-peptide antibodies recognized their cognate protein on whole-cell lysates by immunoblotting. The binding profile of the whole serum of a patient with IPD before the purification procedure is depicted in the left panel. The binding profiles of the eluates of the three immunoaffinity columns (CbpD-pep4, PhtD-pep19, and PhtE-pep40) are depicted in the right panels. Apparent molecular size markers in kilodaltons are shown (MWM). Arrows indicate the reactive bands and their estimated molecular mass nearly consistent with the molecular mass of cognate protein, respectively: CbpD (41 kDa), PhtD (93.5 kDa) and PhtE (114.6 kDa), and PhtA (91.5 kDa) and PhtB (92.1 kDa).