FIGURE 3.

Single cysteine mutants retain cholesterol regulation. A–E, CHO-7 cells were transfected for 24 h with the indicated single cysteine mutants. Cells were then pretreated overnight with compactin (5 μm) and mevalonate (50 μm) and then treated for 8 h with Chol·CD (20 μg of cholesterol/ml), compactin, and mevalonate. Protein was harvested, separated by 10% SDS-PAGE, and then immunoblotted with anti-V5 using GAPDH as a loading control. F, densitometry from A–E. Data are normalized to the untreated condition for each mutant, which is set to 1 and indicated with a dotted line. Data are presented as mean ± S.E. (error bars) from n ≥ 3 experiments.