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. 2015 Oct 3;290(46):27533–27544. doi: 10.1074/jbc.M115.675181

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© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

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FIGURE 5. — Protease protection assays in full-length SM support SM N100 topology model. Protease protection assays were carried out using full-length SM with FLAG tags inserted between residues 53/54 and 93/94. CHO-7 cells were transfected with SM-FLAG(53/54)-V5 or with SM-FLAG(93/94)-V5 for 24 h. Microsomal membranes were isolated and digested with the indicated amount of trypsin. Proteins were separated by 10% SDS-PAGE and immunoblotted with anti-FLAG and anti-Scap R139 antibodies as indicated. * denotes a nonspecific band. Data are representative of n ≥ 3 experiments.