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ITC measurements of nucleotide binding to CBS-PPases. A, typical raw data for successive injections of Ap4A into dhPPase solution. B, integrated heats for titration of four CBS-PPases by selected mono- and dinucleotides after correction for dilution. Enzyme dimer concentration was 4 μm (dhPPase), 5 μm (cnPPase), 3.5 μm (cpPPase), or 2.5 μm (elPPase). The lines show the best fits for a single-binding site model.
