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. 2015 Sep 23;290(46):27594–27603. doi: 10.1074/jbc.M115.680272

TABLE 3.

Kinetic parameters for nucleotide activation derived from the Mg²⁺ dependencies of K_N1 and K_N2 for Ap₃A or K_N for Ap₄A (Fig. 2)

Enzyme	Parameter value									K_m
	Ap₃A						Ap₄A
		K_N1 dependence			K_N2 dependence			K_N,0	K_N,M
		K_N1,0	K_N1,M	K_m	K_N2,0	K_N2,M	K_m	K_N,0	K_N,M
	nm	nm	mm	nm	nm	mm	nm	nm	mm
dhPPase	35 ± 2	96 ± 3	0.7 ± 0.3	75 ± 2	24 ± 1	1.5 ± 0.4	5.5 ± 0.5	5.5 ± 0.5	NA^a
cnPPase	390 ± 10	41 ± 6	3.2 ± 0.7	110 ± 10	14 ± 4	5 ± 3	4.3 ± 0.3	8.6 ± 0.5	0.8 ± 0.3
cpPPase	59,000 ± 5,000	4,200 ± 700	1.4 ± 0.4	5,600 ± 200	640 ± 50	1.1 ± 0.2	1,200 ± 200	55 ± 3	1.4 ± 0.2

^aNA, not attendant.