FIGURE 5.

Stable expression of AtNDT2 reduces proliferation of 293 cells and attenuates cell death induced by inhibition of NAD biosynthesis. A and B, cell proliferation of 293AtNDT2, 293SLC25A32 and 293 parental cells was measured in the absence (A) and presence (B) of 100 μm NA. The results show the averages of three independent experiments (means ± S.D.). C–F, cell proliferation was measured in the indicated cell lines in the absence and presence of the NamPRT inhibitor FK866 (2 μm) or its solvent DMF. The results show the averages of three independent experiments (means ± S.D.).