Figure - PMC

Skip to main content

An official website of the United States government

Here's how you know

Here's how you know

Official websites use .gov
A .gov website belongs to an official government organization in the United States.

Secure .gov websites use HTTPS
A lock ( ) or https:// means you've safely connected to the .gov website. Share sensitive information only on official, secure websites.

View full-text article in PMC

. 2015 Sep 30;290(46):27680–27687. doi: 10.1074/jbc.M115.656173

Search in PMC
Search in PubMed
View in NLM Catalog
Add to search

© 2015 by The American Society for Biochemistry and Molecular Biology, Inc.

PMC Copyright notice

FIGURE 3. — DISC1 knockdown reduces surface and synaptic GABA_AR clusters, whereas DISC1 overexpression increases surface GABA_AR clusters. A and C, immunocytochemical images of surface GABA_AR β2/3 subunits in cortical cultures transfected with a control (con) versus DISC1 shRNA (A) or GFP versus FL-DISC1 (C). Following surface GABA_AR β2/3 labeling, neurons were permeabilized and co-stained with MAP2 (A) or VIAAT (C). Enlarged versions of the boxed regions of dendrites are also shown. B and D, quantitative analysis of surface GABA_AR β2/3 or VIAAT clusters in cortical cultures with different transfections. ***, p < 0.001; **, p < 0.01; *, p < 0.05; Student's t test. Fluo, fluorescence; Norm, normalized. E, immunocytochemical images of control shRNA- versus DISC1 shRNA-transfected cortical neurons co-stained with GABA_AR β2/3 and VIAAT. Enlarged versions of the boxed regions of dendrites are also shown. F, quantitative analysis of the densities of total GABA_AR clusters, VIAAT clusters, and synaptic GABA_AR clusters (co-localized with VIAAT). ***, p < 0.001, Student's t test.