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. 2015 Sep 14;290(46):27736–27748. doi: 10.1074/jbc.M115.673632

FIGURE 4.

FIGURE 4.

Neu5Ac2en binding. a, the NanC active site (yellow) is shown with Neu5Ac2en (green) bound (PDB code 4YW3). Predicted hydrogen bonding interactions are shown with black dashed lines. A hydrophobic interaction between the C9 and Phe-396 is indicated with green and blue spheres (Van der Waals contacts generated using PROBE (79)). The hydrophobic stack providing specificity for α2,3-linked substrates is highlighted with a semitransparent gray surface. b, a schematic representation of the interactions between Neu5Ac2en and the active site. c, for comparison the active sites of NanA (blue) and NanB (purple) are aligned onto NanC. d, a close-up view of the distances in Å between the Neu5Ac2en anomeric carbon and conserved acid base aspartic acid. e, three orientations of the bound Neu5Ac2en ligand are shown. The Fo − Fc omit electron density map is contoured at sigma levels of 3 (cyan), 5 (orange), and 7 (magenta) carved at a distance of 1.6 Å.