FIGURE 8.

CPS1 is necessary for AhR-mediated PADI2 induction. A, schematic depicting the location of NC-XRE sequences (vertical lines) within a 5-kb region upstream of the PADI2 gene transcription start site. B, AML12 cells were grown in the presence and absence of 10 μm 5-aza-2′-deoxycitidine (Aza) and transfected with siRNAs as described in the Fig. 6 legend. For final 24 h, AML12 cells were treated with either vehicle or 6 nm TCDD. Quantitative RT-PCR for PADI2 on isolated total RNA is shown. *, p < 0.05 (n = 3). C, in vivo ChIP and re-ChIP assays for the AhR, CPS1, H3 (positive control), and IgG (negative control) in mouse liver treated with vehicle or TCDD (20 μg/kg for 2 h). Quantitative PCR-amplified the NC-XRE cluster between −3850 and −3550 bp in the PADI2 upstream region.