TABLE 1.

Apparent affinity of wild type and the different mutant PRKAR1A subunits for the PRKACA subunit

n represents the number of experiments. p values are calculated by comparing the results of each mutant with the results for the wild type protein (unpaired Student's t test).

	BRET50 (mean ± S.E.)	n	p
WT	0.48 ± 0.08	5
Y175C	0.40 ± 0.15	3	0.65
A213T	0.22 ± 0.11	3	0.11
A213D	0.40 ± 0.15	4	0.65
Q285R	0.48 ± 0.35	2	0.99
G289E	0.34 ± 0.17	4	0.45
G289W	0.51 ± 0.27	3	0.91
A328V	0.28 ± 0.18	2	0.30
R335L	0.53 ± 0.43	2	0.85
R368X	0.35 ± 0.14	5	0.46
Q372X	0.76 ± 0.19	2	0.17