TABLE 2.
Dissociation of PKA regulatory and catalytic subunits induced by cAMP analogs
| Mutant | 8-Bromo-cAMP |
8-AHA cAMP |
8-PIP cAMP |
||||||
|---|---|---|---|---|---|---|---|---|---|
| EC50 (mean ± S.D.) | n | p versus WT | EC50 (mean ± S.D.) | n | p versus WT | EC50 (mean ± S.D.) | n | p versus WT | |
| nm | nm | nm | |||||||
| WT | 9.0 ± 4.1 | 9 | 1.0 ± 0.4 | 7 | 300.3 ± 135.4 | 8 | |||
| Q372X | 21.2 ± 5.9 | 5 | 0.010 | 8.5 ± 3.2 | 5 | 0.010 | 176.5 ± 36.4 | 5 | 0.050 |
| R368X | 20.9 ± 5.5 | 4 | 0.023 | 12.1 ± 5.6 | 4 | 0.044 | 553.5 ± 182.3 | 3 | 0.177 |
| R335L | 21.1 ± 6.1 | 5 | 0.012 | 64.2 ± 21.9 | 4 | 0.015 | 732.2 ± 1242.0 | 5 | 0.526 |
| A328V | 23.6 ± 7.8 | 5 | 0.016 | 1.8 ± 0.4 | 4 | 0.017 | 374.5 ± 149.7 | 4 | 0.492 |
| G289E | 182.2 ± 41.3 | 6 | <0.001 | 67.7 ± 17.8 | 4 | 0.007 | 3415.1 ± 2091.4 | 7 | 0.011 |
| Q285R | 21.3 ± 5.7 | 5 | 0.008 | 2.4 ± 1.0 | 5 | 0.055 | 191.2 ± 80.6 | 5 | 0.122 |
| A213T | 31.5 ± 13.1 | 6 | 0.011 | 3.3 ± 2.5 | 5 | 0.136 | 1948.0 ± 387.7 | 4 | 0.004 |
| Y175C | 35.3 ± 11.7 | 6 | 0.003 | 2.8 ± 2.3 | 4 | 0.273 | 1303.8 ± 238.8 | 5 | <0.001 |