FIGURE 9.
The loss of rds glycosylation induces subtle alterations in RDS·ROM-1 complex formation cones. Retinal extracts from the nrl−/− (red) and rdsN/N/nrl−/− (blue) were separated on non-reducing 5–20% sucrose gradients, and gradient fractions were subsequently separated by reducing SDS-PAGE/Western blot (A and B) or non-reducing SDS-PAGE/Western blot (C and D). Blots were probed with RDS (A and C) or ROM-1 (B and D). In the quantifications in A and B, nrl−/− values are plotted in blue, and rdsN/N/nrl−/− values are plotted in red. In the quantifications in C and D, nrl−/− and rdsN/N/nrl−/− monomers are plotted in black/green, respectively, whereas dimers are plotted in blue/red, respectively. Shown are means (n = 4) ± S.E. IB, immunoblot.