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. 2015 Sep 18;290(46):27914–27927. doi: 10.1074/jbc.M115.661645

FIGURE 1.

FIGURE 1.

Secreted proteins of A. nidulans grown with sugars and β-mannans as sole carbon source. A, SDS-PAGE analysis of extracellular proteins from A. nidulans grown with glucose (lane 1), mannose (lane 2), galactomannan (lane 3), or glucomannan (lane 4) as the sole carbon sources for 24 h. Culture filtrates (2.5 ml) were concentrated to 25 μl using Amicon Ultra filter units and then analyzed by SDS-PAGE. Protein concentrations in culture filtrates were 10.1 and 9.3 μg/ml with galactomannan and glucomannan, respectively, as the carbon source. Proteins were not detected with glucose and mannose. B, expression profiles of extracellular proteins of A. nidulans grown in glucomannan as a sole carbon source and resolved on two-dimensional gel electrophoresis gels. About 100 μg of crude proteins of A. nidulans grown in MM medium containing glucomannan as the sole carbon source were resolved by two-dimensional gel electrophoresis as described under “Experimental Procedures.” Lane M, protein standard. pI, isoelectric point. Arrows, proteins analyzed by MALDI-TOF/TOF-MS. Identified protein bands are marked by arrows with numbers (Table 2).