FIGURE 11.

Synergistic action of Man134A and Man5C for hydrolysis of β-mannans. A and B, synergy plots of reducing sugar production from glucomannan (A) and galactomannan (B) by Man134A and Man5C (ratios of Man134A:Man5C of 100:0, 90:10, 75:25, 50:50, 25:75, 10:90, 0:100) after incubation for 15 min (circles), 30 min (triangles), 60 min (squares), and 120 min (diamonds). Man134A and Man5C (0.4 μm as a total) were incubated with 1.0% substrate in 50 mm sodium phosphate buffer (pH 6.0) at 37 °C. Dashed lines indicates the theoretical sums of reducing sugar production by the enzymes when there is no synergy.