FIGURE 12.

Effects of Man134A gene disruption on cell growth and β-1,4-mannanase activity. Effect of medium pH on growth of wild type and Man134AΔ strains grown in 1.0% glucomannan (A) or 1.0% glucose (B) as the sole carbon source. A. nidulans strains were cultured in liquid MM medium containing glucomannan or glucose as the sole carbon source at 37 °C for 24 h. Time-dependent changes in dry cell weight (C) and reducing sugar production by β-1,4-mannanase in culture filtrates (D) of A. nidulans strains grown 1.0% glucomannan at pH 6.5 are shown. E, expression profiles of extracellular proteins of WT and Man134AΔ strains. SDS-PAGE of extracellular proteins of WT (lanes 1–4) and Man134AΔ (lanes 5–8) strains grown in 1.0% glucomannan as sole carbon source. A. nidulans strains were cultured at pH 4.0 (lanes 1 and 5), 5.0 (lanes 2 and 6), 6.0 (lanes 3 and 7), and 7.0 (lanes 4 and 8). Crude proteins (25 μg) from 24-h cultures were separated on 12% polyacrylamide gels as described under “Experimental Procedures.”