FIGURE 5.

Hydrolysis of galactose-free β-mannan by purified ANID_02710 product. A, MALDI-TOF-MS analysis of hydrolytic products from galactose-free β-mannan generated by ANID_02710 product. Recombinant ANID_02710 product (1.0 μm) was incubated with 0.5% galactose-free β-mannan in 50 mm sodium phosphate buffer (pH 6.0) at 37 °C for 60 min, and then reaction products were detected as corresponding sodium adducts (molecular weight + 23). B, hydrolysis products of galactose-free β-mannan incubated without (blank) or with recombinant ANID_02710 product for 10–60 min monitored using HPLC. C, TLC analysis of soluble products in reaction mixtures of ANID_02710 product with galactose-free β-mannan. Recombinant ANID_02710 product (1.0 μm) was incubated with 0.5% substrate in 50 mm sodium phosphate buffer (pH 6.0) at 37 °C for 10 to 60 min. M₁, mannose; M₂, mannobiose; M₃, mannotriose; M₄, mannotetraose; M₅, mannopentaose; M₆, mannohexaose. Std, manno-oligosaccharides standard (0.5 mm each).