TABLE 1.

Oligonucleotide primers used in this study

Gene ID numbers are according to the Aspergillus Comparative Database. Restriction sites are underlined.

Primers	Gene	Nucleotide sequence	Application
Real time PCR
RTan2710-f	AN2710	5′-GGAAACACCCTTGATCTTGC-3′
RTan2710-r		5′-AGGTCTTCCCATCACCGTAG-3′
RTan6427-f	AN6427	5′-ACAGTGAACCTGGGAGGACA-3′
RTan6427-r	(Man5C)	5′-TAACGGGTGACCATTTCCTT-3′
RTan9276-f	AN9276	5′-GAGGGAATGACTGGTCTGGA-3′
RTan9276-r		5′-TTGTCGTCGTTGTCGTTGTT-3′
RTan2709-f	AN2709	5′-GCGGAATGAATGCCTACGTT-3′
RTan2709-r		5′-TGCCTCCATCAAGACCCATT-3′
RTao0445-f	AO090038000445	5′-CGGAAAAGCTCGGATGTCTA-3′
RTao0445-r		5′-ATGGACGGCAGACTTGTAGG-3′
Construction of gene disruption cassette
an2710–5-f	AN2710	5′-CCTATTCCAGATTATTGCATTCAAGTT-3′	5′ region of AN2710
an2710–5-r		5′-AAAACCGCGAAATAAAGCTTACTGTTGACTTAAATCTGAATTG-3′
argB-f		5′-CAATTCAGATTTAAGTCAACAGTAAGCTTTATTTCGCGGTTTT-3′	argB
argB-r		5′-CTCCGACTACACCCAAGCTGTCGACCTACAGCCATTGCG-3′
an2710–3-f		5′-GCAATGGCTGTAGGTCGACAGCTTGGGTGTAGTCGGAGAG-3′	3′ region of AN2710
an2710–3-r		5′-CCCCGCCAGGATTCTTAACGCCC-3′
Plasmid for expressing ANID_02710 in A. nidulans
an2710RE-f	AN2710	5′-GCTCTAGACCTATTCCAGATTATTGCATT-3′	pAN2710
an2710RE-r		5′-TTTTCCTTTTGCGGCCGCATATCTGATAGCTCGATGCGATTA-3′
Plasmids for recombinant protein production
an2710-f	AN2710	5′-CCCAAGCTTCGGCCCCCACGACGGACATGACCA-3′	pETAN2710
an2710-r		5′-CCGCTCGAGGATAGCCTGGACATCAACCCAAAAGCG-3′
Man5C-f	AN6427 (Man5C)	5′-CGGGGTACCCGCAAGGGCTTTGTGACCACCAAAGGCGA-3′	pPICZMan5C
Man5C-r		5′-ATAGTTTAGCGGCCGCCTACCGTCTCCGGTTCAACTTGTT-3′