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. 2015 Sep 30;290(46):27972–27985. doi: 10.1074/jbc.M115.668491

FIGURE 7.

FIGURE 7.

Effect of receptor density on concentration-response curves of fusion proteins. A, free MC2R and MRAP and mutant fusion proteins were tested as described for Fig. 2. B and C, cells were transfected using either 3 or 30 ng of receptor DNA/well in a 96-well plate and equivalent amounts in 24-well plates. ACTH responses (B) and surface expression (C) were measured 24 h later. Dashed lines show the lower concentrations of DNA. In all cases, receptor expression was significantly lower with the lower amount of DNA (p < 0.05). Error bars show S.E.