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. Author manuscript; available in PMC: 2015 Nov 16.
Published in final edited form as: Biochemistry. 2015 Sep 17;54(38):5849–5855. doi: 10.1021/acs.biochem.5b00660

Fig. 3. ITC measurement of melibiose binding with MelB in UDM.

Fig. 3

MelB was extracted with UDM and purified in buffer containing 0.035% UDM. Thermograms (with y-axis on the left side) were recorded at 25 °C during the titration of WT MelBSt, mutant R141C MelBSt, or WT MelBEc (80 μM) with melibiose at 10 mM (black curves) or 100 mM (pink curves). Injection of melibiose to the buffer in the absence of a protein is used for the control. Melibiose binding to the WT MelBSt has been reported.12, 16 Accumulated heat change (ΔQ, with y-axis on the right side) of each injection was plotted against the melibiose/MelB molar ratio (on the top), and fitted to a one-site independent binding model.