Fig. 5. Thermogram of IIA^Glc binding.

Titration of WT MelB_Ec (30 μM, at 25 °C) or R141C MelB_St (50 μM, at 20 °C) with IIA^Glc (400 or 455 μM) in the absence or presence of 10 mM melibiose was recorded, respectively. Both MelB were purified in buffer containing 0.035% UDM after extracted with UDM. Injection of IIA^Glc to the buffer in the absence of a protein is used as the control. The K_d for IIA^Glc binding to WT MelB_Ec has been reported.¹⁶