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. Author manuscript; available in PMC: 2015 Nov 16.
Published in final edited form as: Biochemistry. 2015 Sep 17;54(38):5849–5855. doi: 10.1021/acs.biochem.5b00660

Table 1.

IC50 for melibiose displacement of bound D2Ga (mM)

Permease RSO membrane
vesicles
Detergent solubilization

DDM UDM MNG-3 GDN
MelBEc 0.66 ± 0.16b NDc ND 1.20 ± 0.35 1.82 ± 0.18
MelBSt 2.42 ± 0.22 5.65 ± 1.75 4.79 ± 0.71 4.22 ± 0.82 3.19 ± 0.41
R295C MelBSt 4.04 ± 0.42d /e / 5.49 ± 0.83 /
a

Melibiose concentration for the half-maximal displacement of bound D2G (IC50) in the presence of 20 mM NaCl.

b

SEM, (n = 2).

c

No detectable signal.

d

Data from reference #12;

e

No measurement.