Table 2.
Detergent effect on energetics of melibiose binding to MelBa
| Permease |
Ka
(/mol) |
Kd
(mM) |
ΔG
(kJ/mol) |
ΔH (kJ/mol) |
-TΔS
(kJ/mol) |
|---|---|---|---|---|---|
| MelBSt in UDM | 1030 (27.50) | 0.97 (0.02) | −17.20 (0.07) | −10.33 (0.36) | −6.87 (0.28) |
| MelBSt in MNG-3 | 402.18 (20.60) | 2.51 (0.13) | −14.68 (0.13) | −13.65 (0.36) | −1.21 (0.40) |
| MelBEc in UDM | NDc | ND | ND | ND | ND |
| MelBEc in MNG-3 | 782.05 (38.65) | 1.28 (0.06) | −16.51 (0.12) | −9.33 (0.04) | −7.18 (0.16) |
a
All measurements were carried out at 25 °C. The data are presented in Figs. 3 and 4.
b SEM, number of tests = 2 - 4.
c
No detectable signal.