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. Author manuscript; available in PMC: 2016 Oct 1.
Published in final edited form as: Prog Neuropsychopharmacol Biol Psychiatry. 2014 Dec 6;62:61–67. doi: 10.1016/j.pnpbp.2014.11.009

Figure 3.

Figure 3

Cellular localization of 7TM or 6TM MOR isoforms shows. Confocal images of HEK-293 cells expressing 7TM or 6TM MOR: A) 7TM MOR localizes on the cell surface, B) 6TM isoform is mainly localized in intracellular compartments. HEK-293 cells were transiently transfected with plasmids encoding MYC-tagged 7TM or FLAG-tagged 6TM. After forty-eight hours cells were fixed and stained with either Anti-MYC-FITC Antibody or Anti-DYKDDDDK Tag Antibody (Alexa Fluor 488 conjugate); DNA was counterstained with Hoechst 33342. Scale bar: 10 μm.