A. Overnight cultures of PAO1 [WT] and BPA46 [ΔamiB (PTOPLAC::amiB)] were subcultured 1:10 into LB with 1mM IPTG and allowed to grow for 2h at 30°C. They were then normalized to OD600 of 0.5, washed with fresh LB, and subjected to serial dilution. 5µL of each dilution were spotted onto LB plates with or without 1mM IPTG as indicated.
B. Overnight cultures of PAO1 [WT] and BPA157 [ΔamiB] grown in LB supplemented with 5% sucrose were normalized to OD600 of 0.5 and subjected to serial dilution, before 5µL of each dilution were spotted onto LB plates either containing or lacking 5% sucrose. Plates were incubated at 30°C for 2d prior to imaging.