Figure 2. Depletion of AmiB leads to inhibition of growth and aberrant cell morphology.

A. Growth curve of BPA46 [ΔamiB (P_TOPLAC::amiB)] with and without 1mM IPTG. An overnight cell culture was washed with fresh LB and diluted to an OD₆₀₀ of 0.04 in LB either containing or lacking 1mM IPTG. Cells were then grown with agitation at 37°C, with OD₆₀₀ readings taken every 30min. At 2.5h and 5h time points (marked with arrows), the cultures were diluted to prevent entry into stationary phase.

B. At the indicated time points of the growth curve presented in (A), samples of cells grown without IPTG were chemically fixed and preserved on ice. These cells were subsequently imaged using phase contrast optics. The bars equal 2µm.