CYP11B2 expression in human (A–C) and rat (D–F). (A, D) Quantitative RT-PCR analysis for CYP11B2 mRNA across adrenal, brain, and heart tissues. (B, E) Quantitative RT-PCR analysis for CYP11B2 mRNA across ZG and ZF adrenal zones. Values represent mean ± S.E.M. *P<0.05, **P<0.01, ***P<0.001. (C, F) Immunohistochemical analyses of CYP11B2 protein localization in ZG and ZF adrenal zones, plus hematoxylin nuclear counterstain.