KCNK9 (TASK-3) expression in human (A–C) and rat (D–F). (A, D) Quantitative RT-PCR analysis for KCNK9 mRNA across adrenal, brain, and heart tissues. (B, E) Quantitative RT-PCR analysis for KCNK9 mRNA across ZG, ZF, and adrenal medulla. Values represent mean ± S.E.M. *P<0.05, **P<0.01, ***P<0.001. (N.D.) Not detectable by quantitative RT-PCR. (C) Immunohistochemical analyses of KCNK9 protein localization in human ZG and ZF adrenal zones and human adrenal medulla (positive control), plus hematoxylin nuclear counterstain. Note: the immunohistochemistry was performed in human ZG, ZF, and medulla simultaneously under the same conditions. (F) Immunohistochemical analysis of KCNK9 protein localization in rat ZG and ZF adrenal zones, plus hematoxylin nuclear counterstain.