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. Author manuscript; available in PMC: 2016 Dec 5.
Published in final edited form as: Mol Cell Endocrinol. 2015 Sep 12;417:141–148. doi: 10.1016/j.mce.2015.09.011

Figure 4.

Figure 4

KCNJ5 (Kir3.4) expression in human and rat. (A, D) Quantitative RT-PCR analysis for KCNJ5 mRNA across adrenal, brain, and heart tissues. (B, E) Quantitative RT-PCR analysis for KCNJ5 mRNA across ZG and ZF adrenal zones. Values represent mean ± S.E.M. *P<0.05, **P<0.01, ***P<0.001. (N.D.) Not detectable by quantitative RT-PCR. (C) Immunohistochemical analyses of KCNJ5 protein localization in human ZG and ZF adrenal zones, plus hematoxylin nuclear counterstain. (F) Immunohistochemical analysis of KCNJ5 protein localization in rat ZG and ZF adrenal zones and rat heart tissue (positive control), plus hematoxylin nuclear counterstain. Note: the immunohistochemistry was performed in rat adrenal and rat heart simultaneously under the same conditions.