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. Author manuscript; available in PMC: 2015 Nov 16.
Published in final edited form as: Dev Dyn. 2013 Feb 8;242(3):230–253. doi: 10.1002/dvdy.23920

Figure 4. Expression of dorsal and ventral markers reflects Neurog1 expression in vivo.

Figure 4

A. Cells induced in the absence of retinoic acid (RA) demonstrate an anterior neural phenotype (expression of Otx2), whereas a marker of spinal cord (Hoxc6) was not expressed. Cells treated with 1 µM RA show diminished Otx2 expression and increased expression of Hoxc6 indicating that the anterior-posterior identity of the neuronal precursors can be modulated by treatment with RA.

B. Neurog1 is expressed in the ventral half of the developing neural tube, in the roof plate and neural crest-associated structures such as dorsal root ganglia. Pax3 and Pax7 are expressed in the dorsal half of the cord, Dbx2 in the middle third, Nkx6.1 and Nkx2.2 in the ventral spinal cord.

C. With differentiation in either complete ES medium (in which some RA is likely present in the serum), or in defined neural medium supplemented with 1 µM RA, expression of dorsal (Pax3 and Pax7), intermediate (Dbx2), and ventral (Nkx2.2 and 6.1) markers were observed in RT-PCR.