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. Author manuscript; available in PMC: 2015 Nov 16.
Published in final edited form as: Dev Dyn. 2013 Feb 8;242(3):230–253. doi: 10.1002/dvdy.23920

Figure 7. Shift in dorsal-ventral phenotypes with Shh and Nog treatment.

Figure 7

Left panels: Immunostaining with Nkx6.1 (green nuclei) and Pax7 antibodies (red nuclei) indicate that more Pax7+ precursors are present in the absence of or at low doses of Sonic Hedgehog (Shh) and/or Noggin (Nog). Higher doses of Shh and/or Nog reduce the number of Pax7+ cells observed and generate more Nkx6.1+ cells (not shown), and the application of high doses of Shh and Nog together (↑Shh↑Nog) resulted in a dramatic increase in Nkx6.1+ cells and a concomitant decrease in the number of Pax7+ cells. Total cell number in each field was determined by Hoechst nuclear staining (blue). Scale bar = 100 micrometers. Right panels: After cell counts, cultures were stained with Tuj1 to distinguish neurons (red cytoplasm), which are largely Pax7- (red nuclei) and Nkx6.1-(green nuclei) as might be expected for markers that are normally down-regulated with differentiation. Scale bar = 50 micrometers.