Figure 8. Quantification of dorsal-ventral phenotypes with Shh and Nog treatment.
There was a dose-dependent decrease in the percentage of Pax7 + cells and an increase in the percentage of Nkx6.1 positive cells following Nog and Shh treatment. Differences observed between all treatment groups were statistically significant (p ≤ 0.05, ANOVA, followed by pairwise Student’s t-test). n = 1000 cells from 10 random fields from two replicate cultures. ↓Shh = 2.5 nM, ↑Shh = 25 nM, ↓Nog = 2 nM, ↑Nog = 5 nM