FIGURE 7.

PEDF amino acids that modify binding to the P1 fragment of the PEDF receptor. P1 peptide (orange) interacts with the solvent accessible 17-mer peptide region (blue) within the neuroprotective 44-mer peptide region (red). A, the inset shows the close proximity of the positively charged amino acid Arg⁹⁹ (blue) of PEDF and two negatively charged amino acids Glu²³⁰ and Glu²³⁶ (orange) of the LBD of PEDF-R that could be important for binding. B, the molecules are rotated 90° to the right on the y axis and 15° forward on the z axis relative to A. The inset shows the close proximity of the hydrophilic His¹⁰⁵ (blue) to the hydrophobic Leu²²² (orange) in the P1 fragment. Amino acids Arg⁹⁹ and His¹⁰⁵ of PEDF and Glu²³⁰, Glu²³⁶, and Leu²²² are shown with side chains in sticks.