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. 2015 Aug 20;290(42):25322–25332. doi: 10.1074/jbc.M115.645234

FIGURE 3.

FIGURE 3.

hMincle selectively responds to cholesterol. A, chemical structures of cholesterol, steroid hormones, and sterols. B, reactivity of hMincle to cortisone, progesterone, estradiol, testosterone, aldosterone, and DHEA. Each steroid hormone was coated onto a plate (1 μg/well) to stimulate reporter cells expressing FcRγ alone or together with mMincle or hMincle for 18 h. The NFAT-GFP induction was analyzed by flow cytometry. C, responsiveness of hMincle to cholesteryl esters. Plate-coated cholesteryl esters (5 μg/well) were co-cultured with reporter cells expressing FcRγ alone or together with mMincle or hMincle for 18 h. Each ester differs in the length of fatty acid (14:0,16:0, 18:0, 20:0, and 22:0). D, reactivity of hMincle to sterols. Reporter cells expressing FcRγ alone or together with mMincle or hMincle were stimulated with the indicated sterols in a plate-coated form for 18 h. Data are representative of three independent experiments (B–D). Data are presented as mean ± S.D. *, p < 0.05 (B–D).