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. 2015 Aug 20;290(42):25322–25332. doi: 10.1074/jbc.M115.645234

FIGURE 5.

FIGURE 5.

Interaction of hMincle with cholesterol crystals induces the production of pro-inflammatory cytokines. A, RAW-Blue cells expressing hMincle or parental cell lines were stimulated with cholesterol crystals, LPS (10 ng/ml), or TDM (100 ng/well). Supernatants were collected after 24 h of culture. Concentrations of TNF and MIP-2 were determined by ELISA. B, RAW-Blue cells expressing hMincle were stimulated with cholesterol crystals (100 μg/ml), LPS (10 ng/ml), or TDM (100 ng/ml) for 24 h in the presence or absence of the indicated amount of anti-hMincle antibody (anti-hMincle) or isotype control antibody (R3-34) (control mAb). Concentrations of TNF and MIP-2 were determined by ELISA. All data are representative of two independent experiments and presented as mean ± S.D. *, p < 0.05.