FIGURE 5.

Peroxisomal import of Gpd1p does not depend on Pnc1p but import of both proteins is dependent on PTS2 motif of Gpd1p. A, fluorescence microscopy localization of plasmid-encoded Gpd1p-GFP in a Pnc1p-deficient strain. Gpd1p-GFP shows co-localization with the peroxisomal marker mCherry-Ant1p. B, fluorescence microscopy of plasmid-encoded Gpd1p-GFP, Gpd1pΔPTS2-GFP, mCherry-Pnc1p, and the peroxisomal marker protein mCherry-Ant1p in Δgpd1 mutant cells. The data revealed that the peroxisomal localization of Gpd1p and Pnc1p depends on the presence of the PTS2 motif of Gpd1p.