FIGURE 2.

CaMKIIδB localization to the nucleus is increased in CPCs during commitment. A, CaMKIIδB and B, CaMKIIδC mRNA in CPCs with and without dexamethasone (Dex) stimulation for 7 days represented as a fold change relative to CPCs maintained in growth medium (GM). C, CaMKIIδ protein levels in whole cell lysates with and without Dex stimulation. D, quantitation of total CaMKIIδ levels relative to GM-treated CPCs. E, CaMKIIδ protein is primarily cytosolic in CPCs without differentiation stimulus. F, CaMKIIδ increases in expression and localizes to the nuclear compartment of CPCs after 6 days of Dex treatment. G, CaMKIIδ expression in the cytosolic and nuclear fractions of CPCs after induction with differentiation medium. H, quantitation of cytosolic CaMKIIδ and I, nuclear CaMKIIδB. Graphs are represented as a fold change relative to basal CPCs in cytosolic and nuclear fractions. GAPDH is probed as a loading control for immunoblotting. p values compare CPCs in 10% FBS α-MEM without Dex to CPCs treated with Dex.