CaMKIIδB overexpression in CPCs increases cell commitment toward the cardiomyogenic lineage.
A, CPCeδB stimulated with Dex for 6 days show increased expression of cardiogenic marker cardiac troponin T (tnnt3) and B. α-smooth muscle actin based on mRNA expression. C, cardiac marker α-sarcomeric expression and D, quantitation in CPCe and CPCeδB in normal growth conditions (G), −Dex and +Dex. E, metabolic activity under GM, −Dex, or +Dex growth conditions. F, endothelial (Tie2) and smooth muscle (α-SMA) were probed in CPCe and CPCeδB grown in normal growth conditions (G), −Dex or +Dex. G, quantitation of Tie2 and H. α-SMA, which is up-regulated in Dex-treated CPCeδB. Values are represented as relative fluorescent units (RFU) normalized to GAPDH or tubulin. *, p < 0.05 and **, p < 0.01 CPCe versus CPCeδB.