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araf-tv2 overexpression has no effect on caMEK1-induced marker changes. Embryos at the one-cell stage were injected with caMEK1 mRNA alone or together with araf-tv2 mRNA and examined for marker expression at the shield stage. A, expression patterns of marker genes. For chd, eve1, and gata2, embryos were shown in animal-pole view with dorsal to the right; for gsc, embryos were orientated in dorsal view. Scale bar, 200 μm. B, statistical data. n, the total number of observed embryos.
