FIGURE 1.

COS-7 cells were transfected with 4 μg of WT-CFTR or ΔF508. A, expression of ΔF508-CFTR. Immunoblots and graphs comparing the expression of bands B and C in the ΔF508 mutant and WT-CFTR. Versus WT: ##, p < 0.01. B, temperature correction of ΔF508. Immunoblots and graphs comparing the expression of CFTR in cells with ΔF508 incubated in 27 or 37 °C. Versus 37 °C: **, p < 0.01; ***, p < 0.001. C-E, correcting ΔF508-CFTR. Cells were treated 16 h with increasing doses of C3 + C4 (C), C18 + C3 (D), and C18 + C4 (E). Versus 0 μm: *, p < 0.05; **, p < 0.01. Versus 10 μm: ○, p < 0.05 (n = 3). Ezrin (Ez) was used as a loading control.